Ultrastructure study of vitellogenesis of experimentally recovered Fasciola gigantica

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Cholestasis caused by Fasciola gigantica.

Fascioliasis is an infectious disease caused by the hepatic trematodes Fasciola hepatica and Fasciola gigantica. Here, we report the case of Fasciola gigantica presenting with biliary obstruction and abdominal pain that was diagnosed and treated by endoscopic retrograde cholangiography (ERCP). A 46-year-old woman presented with right upper quadrant abdominal pain and jaundice. Physical examinat...

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Determination of somatic and excretory-secretory antigens of Fasciola hepatica and Fasciola gigantica using SDS-PAGE

Human fascioliasis due to unknown species and animal fascioliasis caused by both or one of Fasciola spp. are commonly seen in Iran. To compare electrophoretic patterns of somatic and excretory-secretory antigens of F. hepatica and F. gigantica  by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), the adult flukes were collected from infected slaughtered bovine livers. E/S a...

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Detection of Coproantigens by Sandwich ELISA in Rabbits Experimentally Infected with Fasciola gigantica

BACKGROUND The study was targeted to report the appearance of coproantigens in feces and circulating antibodies in the serum of Fasciola gigantica experimentally infected rabbits. METHODS Copro Hyper Immune Serum (HIS) and Excretory-Secretory Hyper Immune Serum (ES HIS) antigens were used in a sandwich ELISA for the detection of F. gigantica antigens in feces of 12 rabbits experimentally infe...

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Detection of coproantigens by sandwich ELISA in sheep experimentally infected with Fasciola gigantica.

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Fasciola gigantica: immunization of rabbits with proteins isolated from coproantigen.

Two fractions were isolated from coproantigen by ion-exchange chromatography in which DEAE cellulose was utilized. Both fractions and crude antigen were characterized by SDS-polyacrylamide gel electrophoresis which revealed 13 bands of molecular weight ranged from 205-31 in crude coproantigen. While fraction I resolved into six bands of molecular weight 198, 178, 148, 111, 101 & 45. Fraction II...

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ژورنال

عنوان ژورنال: Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology

سال: 2014

ISSN: 2090-0856

DOI: 10.21608/eajbse.2014.14502